full length characterization of pa gene of h9n2 isolated from broilers during 1998 to 2009
نویسندگان
چکیده
background and aims: avian influenza (ai) h9n2 subtype was first reported to infect turkeys in the united states in 1966 and has been panzootic in europe and asia. the impact of avian influenza caused by h9n2 viruses in iran is now significantly more severe than in previous years. methods: sequence analysis and phylogenetic study of the complete coding region polymerase a (pa) gene of h9n2 subtype of avian influenza virus isolates (outbreaks of 1999-2009) in iran were studied. results: sequence and phylogenetic analysis revealed a large number of similar substitution mutations and close evolutionary relation among sequences of pa. based on analysis of aminoacid sequences iranian h9n2 isolates have some substitution that found in human and mouse adapted isolates. it is raised concern that h9n2 isolates has trend to infect mammalian host. in phylogenetic analysis iranian isolates cluster in unknown indian-middle east cluster. however, the early isolate of h9n2 (11t strain) in iran classified in dk-1 lineage. conclusion: the results show, iranian avian influenza h9n2 isolates have undergone extensive genetic reassortment which led to emergence of a new genotype. interestingly, continuous monitoring of viral genetic changes throughout the years is warranted to monitor variations of influenza viruses in the field.
منابع مشابه
Full Length Characterization of PA Gene of H9N2 Isolated from Broilers During 1998 to 2009
Background and Aims: Avian Influenza (AI) H9N2 subtype was first reported to infect turkeys in the United States in 1966 and has been panzootic in Europe and Asia. The impact of avian influenza caused by H9N2 viruses in Iran is now significantly more severe than in previous years. Methods: Sequence analysis and phylogenetic study of the complete coding region Polymerase A (PA) gene of H9N2 subt...
متن کاملPathogenicity and haemagglutinin gene sequence analysis of Iranian avian influenza H9N2 viruses isolated during (1998–2001)
Sixteen avian influenza (AI) H9N2 viruses were isolated from disease outbreaks in different parts of Iranduring (1998–2001). These AI isolates were used for pathogenicity, haemagglutinin (HA) gene variation andphylogenetic analysis. Results in both pathogenicity tests and HA gene cleavage site sequence detectionrepresented a non-highly pathogenic feature for all Iranian AI isolates studied. The...
متن کاملPhylogenetic analysis of PB2 gene of H9N2 subtype of avian influenza viruses isolated from commercial chickens in Tehran province of Iran during 1998-2001
The PB2 protein of influenza A viruses has been shown as host range determinant. In this study PB2gene of five H9N2 isolates from Tehran province during the period of 1998-2001 were partially amplifiedand sequenced to do phylogenetic study. Iranian isolates could be classified in Eurasian lineage according totheir PB2 gene sequences. The PB2 genes of these isolates are different from those of M...
متن کاملpathogenicity and haemagglutinin gene sequence analysis of iranian avian influenza h9n2 viruses isolated during (1998–2001)
sixteen avian influenza (ai) h9n2 viruses were isolated from disease outbreaks in different parts of iranduring (1998–2001). these ai isolates were used for pathogenicity, haemagglutinin (ha) gene variation andphylogenetic analysis. results in both pathogenicity tests and ha gene cleavage site sequence detectionrepresented a non-highly pathogenic feature for all iranian ai isolates studied. the...
متن کاملMolecular characterization of non-structural gene of H9N2 subtype of avian influenza viruses isolated from broiler chickens in Iran
BACKGROUND: The H9N2 subtype of avian influenzaviruses (AIVs) have spread in Asia and Middle East countriesand have become a serious threat to poultry industry in Iran.OBJECTIVES:Characterization of genes of H9N2 subtype involvingin pathogenicity and diagnosis are crucial in control of avianinfluenza outbreaks. The Nonstructural (NS) gene and its proteinproducts (NS1 & NS2) are important as dia...
متن کاملNucleoprotein (NP) Gene Based Phylogenetic Analysis of Iranian H9N2 Avian Influenza Isolates during 1998-2011
Background and Aims: Avian Influenza (AI) H9N2 subtype was first reported to infect turkeys in the United States in 1966 and has been panzootic in Eurasia. In Iran, the H9N2 virus was first isolated from broiler chickens in 1998 in Ghazvin province and it is the most prevalent subtype of influenza virus in poultry industry in Iran at the present time. Materials and Methods: In this study, we se...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of virologyجلد ۵، شماره ۳، صفحات ۱-۶
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023